TB-500
How to reconstitute the 10 mg vial and convert any target dose into insulin units. Reconstitution math and unit conversions for the 10 mg vial. TB-500 has no validated human dose; the usage patterns shown are community-reported and anecdotal.
No validated human dose. TB-500 is a research-use peptide with no FDA-approved dose. The reconstitution math below is exact measurement — a calculator, not advice. Any usage figures are community-reported and clearly labeled, and none of this is medical advice.
Calculate for your vial
Enter the mg on your TB-500 vial, the bacteriostatic water you added, and your target dose — it works out the exact units to draw on a U-100 insulin syringe, for whatever you personally have.
Reconstitution Calculator
Check the decimal. A misplaced decimal point here is a 10× dosing error. Re-read every number, and confirm your dose with a licensed clinician before you draw.
Educational only — not a dosing recommendation. This tool does the measurement math; it does not tell you what to take. On a U-100 insulin syringe, 100 units = 1 mL.
1 · Find your dose
Pick what you're using TB-500 for and how much bacteriostatic water you added — this pulls out the exact units to draw and how often people report using it.
to draw 2.5 mg–2.5 mg at 2 mL water
5 mg/mL · 50 mcg per unit
- How often
- Twice weekly
- Cycle
- ~4–6 weeks, then taper to maintenance
2 · Reconstitute it cleanly, step by step
How to turn the 10 mg powder into a measured liquid with clean, sterile technique. More water means each insulin unit holds less peptide — easier to measure small amounts accurately.
1Swab the stoppers
Wipe the rubber top of each vial with an alcohol pad and let it air-dry.
2Draw the water
Pull your bacteriostatic water up into the insulin syringe.
3Reconstitute
Inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
4Swirl to dissolve
Gently swirl until the powder fully dissolves into a clear liquid. Never shake.
5Equalize, then draw
To draw a dose: push in an equal amount of air first to equalize the pressure, then pull your dose.
- 1
Swab both tops
Wipe the rubber top of the bacteriostatic-water vial and the TB-500 vial stopper with a fresh alcohol pad, and let them air-dry. Never touch the needle or the stoppers after wiping.
Alcohol swab · let dry - 2
Draw the water
First pull 2 mL of air into the syringe and inject it into the bacteriostatic-water vial to equalize the pressure, then draw your 2 mL of water back out. Inject it into the TB-500 vial down the inside glass wall, not onto the powder.
2 mL BAC water - 3
Swirl, don't shake
Gently swirl the 10 mg vial until the powder fully dissolves into a clear liquid. Never shake — shaking can damage the peptide and foam the solution.
Swirl, don't shake - 4
Know your strength
The vial is now 5 mg/mL. Each unit on a U-100 syringe holds about 50 mcg.
5 mg/mL - 5
Re-swab & draw your dose
Wipe the stopper again. With a fresh insulin syringe, pull back 50 units of air and inject it into the vial to equalize the pressure, then draw 50 units (0.5 mL) for a 2.5 mg dose.
50 units - 6
Store it right
Keep the mixed vial in the fridge, away from light. Use a new sterile syringe every time, never share, and drop used sharps in a proper container.
Refrigerate · fresh needle
What each water volume gives you:
3 · Full units reference
Every bacteriostatic-water volume (rows) against every target dose (columns) — each cell is the U-100 units and the exact draw in mL. The highlighted row is the easiest volume to measure.
| BAC water | Concentration | Per unit | 1 mg | 2.5 mg | 5 mg |
|---|---|---|---|---|---|
| 1 mL | 10 mg/mL | 100 mcg | 10u0.1 mL | 25u0.25 mL | 50u0.5 mL |
| 2 mLeasy pick | 5 mg/mL | 50 mcg | 20u0.2 mL | 50u0.5 mL | 100u1 mL |
| 3 mL | 3.33 mg/mL | 33.33 mcg | 30u0.3 mL | 75u0.75 mL | 150u1.5 mL |
| 5 mL | 2 mg/mL | 20 mcg | 50u0.5 mL | 125u1.25 mL | 250u2.5 mL |
4 · Everyday usage
TB-500 is a research peptide with no validated human dose. The patterns below describe what people commonly report — a loading block of roughly 2.5 mg twice weekly (≈5 mg/wk) is the most-cited starting point — not a protocol to follow.
Loading phase
Community-reported · anecdotalAn initial higher-frequency block that community write-ups describe for the first few weeks — commonly ~2.5 mg twice weekly (≈5 mg/wk).
- Reported amount
- 2.5 mg–2.5 mg≈ 50–50 units @ 2 mL
- Frequency
- Twice weekly
- Cycle
- ~4–6 weeks, then taper to maintenance
Community-reported and anecdotal — not from controlled human trials, not a recommendation, and not medical advice.
Maintenance
Community-reported · anecdotalA lower-frequency phase described after the loading block.
- Reported amount
- 2 mg–2.5 mg≈ 40–50 units @ 2 mL
- Frequency
- Once weekly or every other week
Community-reported and anecdotal — not from controlled human trials, not a recommendation, and not medical advice.
Frequently asked questions
How many insulin units is 1 mg of TB-500 from a 10 mg vial?
- Reconstituting a 10 mg vial with 2 mL of bacteriostatic water gives 5 mg/mL — about 50 mcg per unit. Drawing 1 mg is 0.2 mL, or 20 units on a U-100 insulin syringe. This is measurement math, not a dose recommendation.
How many insulin units is 2.5 mg of TB-500 from a 10 mg vial?
- Reconstituting a 10 mg vial with 2 mL of bacteriostatic water gives 5 mg/mL — about 50 mcg per unit. Drawing 2.5 mg is 0.5 mL, or 50 units on a U-100 insulin syringe. This is measurement math, not a dose recommendation.
Is there an established dose of TB-500?
- No. TB-500 (a synthetic fragment related to thymosin beta-4) is a research chemical with no FDA approval and no validated human dose. The figures here are community-reported and anecdotal, shown only to describe reported practice. Dosing decisions belong with a licensed clinician.
WikiPeps is a community reference. Reconstitution figures are deterministic measurement math; usage figures are sourced and labeled. Nothing here is medical advice, a recommendation, or an offer to sell peptides — dosing decisions belong with a licensed clinician.