NAD+
How to reconstitute the 250 mg vial and convert any target dose into insulin units. Reconstitution math and unit conversions for the 250 mg and 500 mg vials. NAD+ is a coenzyme, not an approved drug; clinic use is mainly slow IV infusion and there is no validated dose — figures shown are community-reported.
No validated human dose. NAD+ is a research-use peptide with no FDA-approved dose. The reconstitution math below is exact measurement — a calculator, not advice. Any usage figures are community-reported and clearly labeled, and none of this is medical advice.
Calculate for your vial
Enter the mg on your NAD+ vial, the bacteriostatic water you added, and your target dose — it works out the exact units to draw on a U-100 insulin syringe, for whatever you personally have.
Reconstitution Calculator
Check the decimal. A misplaced decimal point here is a 10× dosing error. Re-read every number, and confirm your dose with a licensed clinician before you draw.
Educational only — not a dosing recommendation. This tool does the measurement math; it does not tell you what to take. On a U-100 insulin syringe, 100 units = 1 mL.
1 · Find your dose
Pick what you're using NAD+ for and how much bacteriostatic water you added — this pulls out the exact units to draw and how often people report using it.
IL-2 (1×10\^6IU) will be injected subcutaneously every other day for 2 weeks (7 times), with an interval of 2 weeks.
- How / how often
- Route: subcutaneous
Observational human-trial dose — studied, NOT a target or recommendation. Phase 3 (NCT05631717). Source: https://clinicaltrials.gov/study/NCT05631717
At 5 mL this 250 mg vial is 50 mg/mL (500 mcg per unit). Use the full matrix below to convert a specific amount into U-100 units — measurement math, not a recommendation.
2 · Reconstitute it cleanly, step by step
How to turn the 250 mg powder into a measured liquid with clean, sterile technique. More water means each insulin unit holds less peptide — easier to measure small amounts accurately.
1Swab the stoppers
Wipe the rubber top of each vial with an alcohol pad and let it air-dry.
2Draw the water
Pull your bacteriostatic water up into the insulin syringe.
3Reconstitute
Inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
4Swirl to dissolve
Gently swirl until the powder fully dissolves into a clear liquid. Never shake.
5Equalize, then draw
To draw a dose: push in an equal amount of air first to equalize the pressure, then pull your dose.
- 1
Swab both tops
Wipe the rubber top of the bacteriostatic-water vial and the NAD+ vial stopper with a fresh alcohol pad, and let them air-dry. Never touch the needle or the stoppers after wiping.
Alcohol swab · let dry - 2
Draw the water
First pull 5 mL of air into the syringe and inject it into the bacteriostatic-water vial to equalize the pressure, then draw your 5 mL of water back out. Inject it into the NAD+ vial down the inside glass wall, not onto the powder.
5 mL BAC water - 3
Swirl, don't shake
Gently swirl the 250 mg vial until the powder fully dissolves into a clear liquid. Never shake — shaking can damage the peptide and foam the solution.
Swirl, don't shake - 4
Know your strength
The vial is now 50 mg/mL. Each unit on a U-100 syringe holds about 500 mcg.
50 mg/mL - 5
Re-swab & draw your dose
Wipe the stopper again. With a fresh insulin syringe, pull back 50 units of air and inject it into the vial to equalize the pressure, then draw 50 units (0.5 mL) for a 25 mg dose.
50 units - 6
Store it right
Keep the mixed vial in the fridge, away from light. Use a new sterile syringe every time, never share, and drop used sharps in a proper container.
Refrigerate · fresh needle
What each water volume gives you:
3 · Full units reference
Every bacteriostatic-water volume (rows) against every target dose (columns) — each cell is the U-100 units and the exact draw in mL. The highlighted row is the easiest volume to measure.
| BAC water | Concentration | Per unit | 10 mg | 25 mg | 50 mg |
|---|---|---|---|---|---|
| 2 mL | 125 mg/mL | 1250 mcg | 8u0.08 mL | 20u0.2 mL | 40u0.4 mL |
| 3 mL | 83.33 mg/mL | 833.33 mcg | 12u0.12 mL | 30u0.3 mL | 60u0.6 mL |
| 5 mLeasy pick | 50 mg/mL | 500 mcg | 20u0.2 mL | 50u0.5 mL | 100u1 mL |
4 · Everyday usage
NAD+ is a coenzyme, not an approved drug for anti-aging or performance. Most clinic use is IV (slow infusion); subcutaneous injection is community practice and is notoriously stingy. There is no validated dose for these uses.
Studied: Systemic Lupus Erythematosus
Studied in trialsIL-2 (1×10\^6IU) will be injected subcutaneously every other day for 2 weeks (7 times), with an interval of 2 weeks.
- Frequency
- Route: subcutaneous
Observational human-trial dose — studied, NOT a target or recommendation. Phase 3 (NCT05631717). Source: https://clinicaltrials.gov/study/NCT05631717
Studied: Renal Insufficiency, Acute
Studied in trials200mg IV Minocin given over 1 hour
- Frequency
- Route: intravenous
Observational human-trial dose — studied, NOT a target or recommendation. Phase 1 (NCT02808052). Source: https://clinicaltrials.gov/study/NCT02808052
Studied: Peripheral Arterial Disease
Studied in trials40 Mg.
- Frequency
- EARLY_Phase 1
Observational human-trial dose — studied, NOT a target or recommendation. EARLY_Phase 1 (NCT00720577). Source: https://clinicaltrials.gov/study/NCT00720577
Wellness use (community)
Community-reported · anecdotalClinics typically give NAD+ as a slow IV drip; some people report small subcutaneous doses at home. The amount is far larger (milligrams) than most peptides.
- Reported amount
- 25 mg–100 mg≈ 50–200 units @ 5 mL
- Frequency
- IV: per clinic protocol · SC: 1–2× weekly (community)
- Cycle
- Often given in short blocks of sessions (community)
Community-reported and anecdotal. NAD+ is an endogenous coenzyme, not an FDA-approved drug for anti-aging or performance; the dominant clinic ROUTE is slow intravenous infusion (rapid IV and SC injection are commonly reported as uncomfortable). No validated dose exists for these uses — this is not a recommendation.
Frequently asked questions
How many insulin units is 10 mg of NAD+ from a 250 mg vial?
- Reconstituting a 250 mg vial with 5 mL of bacteriostatic water gives 50 mg/mL — about 500 mcg per unit. Drawing 10 mg is 0.2 mL, or 20 units on a U-100 insulin syringe. This is measurement math, not a dose recommendation.
How many insulin units is 25 mg of NAD+ from a 250 mg vial?
- Reconstituting a 250 mg vial with 5 mL of bacteriostatic water gives 50 mg/mL — about 500 mcg per unit. Drawing 25 mg is 0.5 mL, or 50 units on a U-100 insulin syringe. This is measurement math, not a dose recommendation.
How is NAD+ usually given, and is there a standard dose?
- Most clinic NAD+ is given as a slow intravenous infusion, not an injection, because faster delivery is often uncomfortable; some people report small subcutaneous doses at home. NAD+ is a natural coenzyme rather than an approved drug for anti-aging or performance, so there is no validated dose — the figures here are community-reported, and decisions belong with a licensed clinician.
References
WikiPeps is a community reference. Reconstitution figures are deterministic measurement math; usage figures are sourced and labeled. Nothing here is medical advice, a recommendation, or an offer to sell peptides — dosing decisions belong with a licensed clinician.