NAD+
How to reconstitute the 500 mg vial and convert any target dose into insulin units. Reconstitution math and unit conversions for the 250 mg and 500 mg vials. NAD+ is a coenzyme, not an approved drug; clinic use is mainly slow IV infusion and there is no validated dose — figures shown are community-reported.
No validated human dose. NAD+ is a research-use peptide with no FDA-approved dose. The reconstitution math below is exact measurement — a calculator, not advice. Any usage figures are community-reported and clearly labeled, and none of this is medical advice.
Calculate for your vial
Enter the mg on your NAD+ vial, the bacteriostatic water you added, and your target dose — it works out the exact units to draw on a U-100 insulin syringe, for whatever you personally have.
Reconstitution Calculator
Check the decimal. A misplaced decimal point here is a 10× dosing error. Re-read every number, and confirm your dose with a licensed clinician before you draw.
Educational only — not a dosing recommendation. This tool does the measurement math; it does not tell you what to take. On a U-100 insulin syringe, 100 units = 1 mL.
1 · Find your dose
Pick what you're using NAD+ for and how much bacteriostatic water you added — this pulls out the exact units to draw and how often people report using it.
to draw 25 mg–100 mg at 5 mL water
100 mg/mL · 1000 mcg per unit
- How often
- IV: per clinic protocol · SC: 1–2× weekly (community)
- Cycle
- Often given in short blocks of sessions (community)
2 · Reconstitute it cleanly, step by step
How to turn the 500 mg powder into a measured liquid with clean, sterile technique. More water means each insulin unit holds less peptide — easier to measure small amounts accurately.
1Swab the stoppers
Wipe the rubber top of each vial with an alcohol pad and let it air-dry.
2Draw the water
Pull your bacteriostatic water up into the insulin syringe.
3Reconstitute
Inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
4Swirl to dissolve
Gently swirl until the powder fully dissolves into a clear liquid. Never shake.
5Equalize, then draw
To draw a dose: push in an equal amount of air first to equalize the pressure, then pull your dose.
- 1
Swab both tops
Wipe the rubber top of the bacteriostatic-water vial and the NAD+ vial stopper with a fresh alcohol pad, and let them air-dry. Never touch the needle or the stoppers after wiping.
Alcohol swab · let dry - 2
Draw the water
First pull 5 mL of air into the syringe and inject it into the bacteriostatic-water vial to equalize the pressure, then draw your 5 mL of water back out. Inject it into the NAD+ vial down the inside glass wall, not onto the powder.
5 mL BAC water - 3
Swirl, don't shake
Gently swirl the 500 mg vial until the powder fully dissolves into a clear liquid. Never shake — shaking can damage the peptide and foam the solution.
Swirl, don't shake - 4
Know your strength
The vial is now 100 mg/mL. Each unit on a U-100 syringe holds about 1000 mcg.
100 mg/mL - 5
Re-swab & draw your dose
Wipe the stopper again. With a fresh insulin syringe, pull back 25 units of air and inject it into the vial to equalize the pressure, then draw 25 units (0.25 mL) for a 25 mg dose.
25 units - 6
Store it right
Keep the mixed vial in the fridge, away from light. Use a new sterile syringe every time, never share, and drop used sharps in a proper container.
Refrigerate · fresh needle
What each water volume gives you:
3 · Full units reference
Every bacteriostatic-water volume (rows) against every target dose (columns) — each cell is the U-100 units and the exact draw in mL. The highlighted row is the easiest volume to measure.
| BAC water | Concentration | Per unit | 10 mg | 25 mg | 50 mg |
|---|---|---|---|---|---|
| 2 mL | 250 mg/mL | 2500 mcg | 4u0.04 mL | 10u0.1 mL | 20u0.2 mL |
| 3 mL | 166.67 mg/mL | 1666.67 mcg | 6u0.06 mL | 15u0.15 mL | 30u0.3 mL |
| 5 mLeasy pick | 100 mg/mL | 1000 mcg | 10u0.1 mL | 25u0.25 mL | 50u0.5 mL |
4 · Everyday usage
NAD+ is a coenzyme, not an approved drug for anti-aging or performance. Most clinic use is IV (slow infusion); subcutaneous injection is community practice and is notoriously stingy. There is no validated dose for these uses.
Wellness use (community)
Community-reported · anecdotalClinics typically give NAD+ as a slow IV drip; some people report small subcutaneous doses at home. The amount is far larger (milligrams) than most peptides.
- Reported amount
- 25 mg–100 mg≈ 25–100 units @ 5 mL
- Frequency
- IV: per clinic protocol · SC: 1–2× weekly (community)
- Cycle
- Often given in short blocks of sessions (community)
Community-reported and anecdotal. NAD+ is an endogenous coenzyme, not an FDA-approved drug for anti-aging or performance; the dominant clinic ROUTE is slow intravenous infusion (rapid IV and SC injection are commonly reported as uncomfortable). No validated dose exists for these uses — this is not a recommendation.
Frequently asked questions
How many insulin units is 10 mg of NAD+ from a 500 mg vial?
- Reconstituting a 500 mg vial with 5 mL of bacteriostatic water gives 100 mg/mL — about 1000 mcg per unit. Drawing 10 mg is 0.1 mL, or 10 units on a U-100 insulin syringe. This is measurement math, not a dose recommendation.
How many insulin units is 25 mg of NAD+ from a 500 mg vial?
- Reconstituting a 500 mg vial with 5 mL of bacteriostatic water gives 100 mg/mL — about 1000 mcg per unit. Drawing 25 mg is 0.25 mL, or 25 units on a U-100 insulin syringe. This is measurement math, not a dose recommendation.
How is NAD+ usually given, and is there a standard dose?
- Most clinic NAD+ is given as a slow intravenous infusion, not an injection, because faster delivery is often uncomfortable; some people report small subcutaneous doses at home. NAD+ is a natural coenzyme rather than an approved drug for anti-aging or performance, so there is no validated dose — the figures here are community-reported, and decisions belong with a licensed clinician.
WikiPeps is a community reference. Reconstitution figures are deterministic measurement math; usage figures are sourced and labeled. Nothing here is medical advice, a recommendation, or an offer to sell peptides — dosing decisions belong with a licensed clinician.